Tech-method Course:

Proteomics

Date

3-4-5 March 2026

Organizers

Angela Bachi (IFOM) & Tiziana Bonaldi (IEO)

Location

IEO Campus, Via Adamello 16 - Milan

About the Course

This course introduces the fundamentals of proteomics while fostering a critical understanding of its most advanced biomedical applications. Students will learn core analytical principles, experimental design, and data interpretation, developing the ability to evaluate emerging proteomic technologies. Emphasis is placed on appreciating both the immense potential and the current challenges of proteomics in biomedical research.

Lessons Location: Coral Room

9:15 – 9:30
Course introduction
A. Bachi/T. Bonaldi

9:30 – 11:00
Introduction to proteomics MS
A. Bachi – IFOM

11:15 – 12:45
Quantitative Proteomics/Expression proteomics with reference to data analysis
A. Cuomo – IEO

14:00 – 15:30
Challenges in PTMs analysis
T. Bonaldi – IEO

15:45 – 17:00
Interaction proteomics & proximity proteomics
T. Bonaldi – IEO

Lessons Location: Coral Room

9:15 – 10:30
Single cell & Spatial Proteomics (super-sensitive sample prep + MS and MS and Ab strengths/caveats)
A. Bachi – IFOM

10:45 – 12:30
Proteomics in clinical research & Drug Discovery, with some examples on HT & automation
R. Noberini – IEO

14:00 – 15:00
The impact of AI in proteomics
A. Vai – IEO

15:15 – 17:00
Mastering the Experimental System: from wet lab preparation to data interpretation 
M. Murgia – University of Padua & Max Planck Institute of Biochemistry, Martinsried

Lessons Location: Coral Room

9:15 – 12:15
Data Analysis Tutorial #1 
A. Cuomo – IEO

13:30 – 14:30
Spatial Proteomics & Data Analysis
D. Schapiro – IFOM & University of Heidelberg

14:45 – 17:30
Data Analysis, Statistics and Functional Annotation Tutorial #2
A. Cuomo – IEO + Matafora IFOM

17:30
Closing Remarks
A. Bachi/T. Bonaldi